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Ted Pella eponate12 resin
Eponate12 Resin, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eponate12+resin/12+eponate+resin/pmc12993172-72-11-13
Average 86 stars, based on 1 article reviews
eponate12 resin - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Incubation:

Article Title: Asymmetric centromere and gene locus positioning in Drosophila neural stem cells
Article Snippet: 72 to 96 h after egg laying old larval brains were dissected using microdissection scissors (Fine Science Tools, catalogue no. 15003-08) and forceps (Dumont #5, Electron Microscopy Sciences, item number 0103-5-PO) in Schneider’s medium supplemented with 1% bovine growth serum (HyClone, item number SH30541.03) and transferred to chambered slides (Ibidi, catalogue no. 80826) for imaging. .. Fixed samples were rinsed with 0.1M cacodylate buffer, post fixed with 1% osmium tetroxide/0.8% potassium ferricyanide for 1 h at 4 °C, rinsed with cacodylate buffer, and incubated in 0.2% tannic acid for 15 min, rinsed with water and en bloc stained with 1% uranyl acetate for 1 h. The samples were then rinsed with distilled water and dehydrated through a graded series of alcohols followed by propylene oxide and embedded in Eponate12 resin (Ted Pella, Inc, Redding, CA). .. Both live and fixed samples were imaged with an Intelligent Imaging Innovations (3i) spinning disk confocal system, consisting of a Yokogawa CSU-W1 spinning disk unit and two Prime 95B Scientific CMOS cameras and a 60x/1.4NA oil immersion objective.

Staining:

Article Title: Asymmetric centromere and gene locus positioning in Drosophila neural stem cells
Article Snippet: 72 to 96 h after egg laying old larval brains were dissected using microdissection scissors (Fine Science Tools, catalogue no. 15003-08) and forceps (Dumont #5, Electron Microscopy Sciences, item number 0103-5-PO) in Schneider’s medium supplemented with 1% bovine growth serum (HyClone, item number SH30541.03) and transferred to chambered slides (Ibidi, catalogue no. 80826) for imaging. .. Fixed samples were rinsed with 0.1M cacodylate buffer, post fixed with 1% osmium tetroxide/0.8% potassium ferricyanide for 1 h at 4 °C, rinsed with cacodylate buffer, and incubated in 0.2% tannic acid for 15 min, rinsed with water and en bloc stained with 1% uranyl acetate for 1 h. The samples were then rinsed with distilled water and dehydrated through a graded series of alcohols followed by propylene oxide and embedded in Eponate12 resin (Ted Pella, Inc, Redding, CA). .. Both live and fixed samples were imaged with an Intelligent Imaging Innovations (3i) spinning disk confocal system, consisting of a Yokogawa CSU-W1 spinning disk unit and two Prime 95B Scientific CMOS cameras and a 60x/1.4NA oil immersion objective.

Article Title: Transcriptional co-activator regulates melanocyte differentiation and oncogenesis by integrating cAMP and MAPK/ERK pathways
Article Snippet: .. B16F1 cells were fixed in a solution of 2% paraformaldehyde, 2.5% glutaraldehyde, and 2mM CaCl 2 in 0.1M sodium cacodylate buffer (pH 7.4) for 2 h at room temperature, post-fixed in 1% osmium tetroxide for 40 and 1.5% potassium ferrocyanide in sodium cacodylate buffer for 1 hour at 4°C in the dark, stained in 1% aqueous uranyl acetate at 4°C in the dark, dehydrated in ethanol graded series, and embedded in Eponate12 resin (Ted Pella). .. Ultra-thin sections of 70 nm were obtained using a diamond knife (Diatome) in an ultramicrotome (Leica EM UC7) and placed on copper grids (300 mesh).

Transmission Electron Microscopy:

Article Title: A Machine Learning–3D Microvessel Platform Identifies Kinase Targets Restoring Blood-Brain-Barrier Endothelial Integrity
Article Snippet: Dried samples were then mounted on stubs, sputter-coated with gold/palladium (Denton Desk IV, Denton Vacuum, Moorestown, NJ), and imaged using a JSM 6610 LV scanning electron microscope at 5 kV (JEOL, Tokyo, Japan). .. For TEM sample preparation, the collagen constructs were rinsed in 0.1 M cacodylate buffer, treated with 1% osmium tetroxide for 2 hours at 4°C, rinsed again with cacodylate buffer, dehydrated through a graded series of ethanol and propylene oxide, and embedded in Eponate12 resin (Ted Pella, Inc, Redding, CA). .. Ultrathin sections (70 nm) were cut using a Leica EM UC7 ultramicrotome, contrasted with uranyl acetate and lead citrate, and imaged on a ThermoFisher Talos L120c transmission electron microscope at 120 kV.

Sample Prep:

Article Title: A Machine Learning–3D Microvessel Platform Identifies Kinase Targets Restoring Blood-Brain-Barrier Endothelial Integrity
Article Snippet: Dried samples were then mounted on stubs, sputter-coated with gold/palladium (Denton Desk IV, Denton Vacuum, Moorestown, NJ), and imaged using a JSM 6610 LV scanning electron microscope at 5 kV (JEOL, Tokyo, Japan). .. For TEM sample preparation, the collagen constructs were rinsed in 0.1 M cacodylate buffer, treated with 1% osmium tetroxide for 2 hours at 4°C, rinsed again with cacodylate buffer, dehydrated through a graded series of ethanol and propylene oxide, and embedded in Eponate12 resin (Ted Pella, Inc, Redding, CA). .. Ultrathin sections (70 nm) were cut using a Leica EM UC7 ultramicrotome, contrasted with uranyl acetate and lead citrate, and imaged on a ThermoFisher Talos L120c transmission electron microscope at 120 kV.

Construct:

Article Title: A Machine Learning–3D Microvessel Platform Identifies Kinase Targets Restoring Blood-Brain-Barrier Endothelial Integrity
Article Snippet: Dried samples were then mounted on stubs, sputter-coated with gold/palladium (Denton Desk IV, Denton Vacuum, Moorestown, NJ), and imaged using a JSM 6610 LV scanning electron microscope at 5 kV (JEOL, Tokyo, Japan). .. For TEM sample preparation, the collagen constructs were rinsed in 0.1 M cacodylate buffer, treated with 1% osmium tetroxide for 2 hours at 4°C, rinsed again with cacodylate buffer, dehydrated through a graded series of ethanol and propylene oxide, and embedded in Eponate12 resin (Ted Pella, Inc, Redding, CA). .. Ultrathin sections (70 nm) were cut using a Leica EM UC7 ultramicrotome, contrasted with uranyl acetate and lead citrate, and imaged on a ThermoFisher Talos L120c transmission electron microscope at 120 kV.

Transmission Assay:

Article Title: Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo.
Article Snippet: .. Pellets were then embedded in Eponate12 resin (TedPella, Inc) 70 nmultrathin sectionswerecut using a Leica EM UC7 ultramicrotome, contrasted with uranyl acetate and lead citrate, and imaged on a Thermo Fisher Talos L120c transmission electron microscope at 120 kV. ..

Microscopy:

Article Title: Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo.
Article Snippet: .. Pellets were then embedded in Eponate12 resin (TedPella, Inc) 70 nmultrathin sectionswerecut using a Leica EM UC7 ultramicrotome, contrasted with uranyl acetate and lead citrate, and imaged on a Thermo Fisher Talos L120c transmission electron microscope at 120 kV. ..



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